diff --git a/arch/arm/src/kinetis/kinetis_lpserial.c b/arch/arm/src/kinetis/kinetis_lpserial.c
index 177a4a7e6be..46908022296 100644
--- a/arch/arm/src/kinetis/kinetis_lpserial.c
+++ b/arch/arm/src/kinetis/kinetis_lpserial.c
@@ -849,6 +849,12 @@ static int kinetis_ioctl(struct file *filep, int cmd, unsigned long arg)
 #ifdef CONFIG_KINETIS_UART_SINGLEWIRE
     case TIOCSSINGLEWIRE:
       {
+        if ((arg & SER_SINGLEWIRE_PULLUP) != 0)
+          {
+            ret = -EINVAL; // Not supported
+            break;
+          }
+
         /* Change to single-wire operation. the RXD pin is disconnected from
          * the UART and the UART implements a half-duplex serial connection.
          * The UART uses the TXD pin for both receiving and transmitting
@@ -856,7 +862,7 @@ static int kinetis_ioctl(struct file *filep, int cmd, unsigned long arg)
 
         regval = kinetis_serialin(priv, KINETIS_LPUART_CTRL_OFFSET);
 
-        if (arg == SER_SINGLEWIRE_ENABLED)
+        if ((arg & SER_SINGLEWIRE_ENABLED) != 0)
           {
             regval |= (LPUART_CTRL_LOOPS | LPUART_CTRL_RSRC);
           }
diff --git a/arch/arm/src/kinetis/kinetis_serial.c b/arch/arm/src/kinetis/kinetis_serial.c
index d5e8890b343..c9f4d944fb4 100644
--- a/arch/arm/src/kinetis/kinetis_serial.c
+++ b/arch/arm/src/kinetis/kinetis_serial.c
@@ -1304,6 +1304,12 @@ static int up_ioctl(struct file *filep, int cmd, unsigned long arg)
 #ifdef CONFIG_KINETIS_UART_SINGLEWIRE
     case TIOCSSINGLEWIRE:
       {
+        if ((arg & SER_SINGLEWIRE_PULLUP) != 0)
+          {
+            ret = -EINVAL; // Not supported
+            break;
+          }
+
         /* Change to single-wire operation. the RXD pin is disconnected from
          * the UART and the UART implements a half-duplex serial connection.
          * The UART uses the TXD pin for both receiving and transmitting
@@ -1311,7 +1317,7 @@ static int up_ioctl(struct file *filep, int cmd, unsigned long arg)
 
         regval = up_serialin(priv, KINETIS_UART_C1_OFFSET);
 
-        if (arg == SER_SINGLEWIRE_ENABLED)
+        if ((arg & SER_SINGLEWIRE_ENABLED) != 0)
           {
             regval |= (UART_C1_LOOPS | UART_C1_RSRC);
           }
diff --git a/arch/arm/src/stm32/stm32_serial.c b/arch/arm/src/stm32/stm32_serial.c
index 2227bc4f418..9afe350cce4 100644
--- a/arch/arm/src/stm32/stm32_serial.c
+++ b/arch/arm/src/stm32/stm32_serial.c
@@ -1952,7 +1952,7 @@ static int up_ioctl(struct file *filep, int cmd, unsigned long arg)
         uint32_t cr = up_serialin(priv, STM32_USART_CR3_OFFSET);
 
 #if defined(CONFIG_STM32_STM32F10XX)
-        if (arg == SER_SINGLEWIRE_ENABLED)
+        if ((arg & SER_SINGLEWIRE_ENABLED) != 0)
           {
             stm32_configgpio((priv->tx_gpio & ~(GPIO_CNF_MASK)) | GPIO_CNF_AFOD);
             cr |= USART_CR3_HDSEL;
@@ -1963,14 +1963,15 @@ static int up_ioctl(struct file *filep, int cmd, unsigned long arg)
             cr &= ~USART_CR3_HDSEL;
           }
 #else
-        if (arg == SER_SINGLEWIRE_ENABLED)
+        if ((arg & SER_SINGLEWIRE_ENABLED) != 0)
           {
-            stm32_configgpio(priv->tx_gpio | GPIO_OPENDRAIN);
+            uint32_t gpio_val = (arg & SER_SINGLEWIRE_PULLUP) ? GPIO_PULLUP : GPIO_OPENDRAIN;
+            stm32_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | gpio_val);
             cr |= USART_CR3_HDSEL;
           }
         else
           {
-            stm32_configgpio(priv->tx_gpio | GPIO_PUSHPULL);
+            stm32_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | GPIO_PUSHPULL);
             cr &= ~USART_CR3_HDSEL;
           }
 #endif
diff --git a/arch/arm/src/stm32f0l0g0/stm32_serial_v1.c b/arch/arm/src/stm32f0l0g0/stm32_serial_v1.c
index 97bf3fbe2d4..44801cad12a 100644
--- a/arch/arm/src/stm32f0l0g0/stm32_serial_v1.c
+++ b/arch/arm/src/stm32f0l0g0/stm32_serial_v1.c
@@ -1602,14 +1602,15 @@ static int stm32serial_ioctl(FAR struct file *filep, int cmd,
 
         uint32_t cr = stm32serial_getreg(priv, STM32_USART_CR3_OFFSET);
 
-        if (arg == SER_SINGLEWIRE_ENABLED)
+        if ((arg & SER_SINGLEWIRE_ENABLED) != 0)
           {
-            stm32_configgpio(priv->tx_gpio | GPIO_OPENDRAIN);
+            uint32_t gpio_val = (arg & SER_SINGLEWIRE_PULLUP) ? GPIO_PULLUP : GPIO_OPENDRAIN;
+            stm32_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | gpio_val);
             cr |= USART_CR3_HDSEL;
           }
         else
           {
-            stm32_configgpio(priv->tx_gpio | GPIO_PUSHPULL);
+            stm32_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | GPIO_PUSHPULL);
             cr &= ~USART_CR3_HDSEL;
           }
 
diff --git a/arch/arm/src/stm32f0l0g0/stm32_serial_v2.c b/arch/arm/src/stm32f0l0g0/stm32_serial_v2.c
index 5ea9c2fc037..8abaf03163a 100644
--- a/arch/arm/src/stm32f0l0g0/stm32_serial_v2.c
+++ b/arch/arm/src/stm32f0l0g0/stm32_serial_v2.c
@@ -1161,14 +1161,15 @@ static int up_ioctl(struct file *filep, int cmd, unsigned long arg)
 
         uint32_t cr = up_serialin(priv, STM32_USART_CR3_OFFSET);
 
-        if (arg == SER_SINGLEWIRE_ENABLED)
+        if ((arg & SER_SINGLEWIRE_ENABLED) != 0)
           {
-            stm32_configgpio(priv->tx_gpio | GPIO_OPENDRAIN);
+            uint32_t gpio_val = (arg & SER_SINGLEWIRE_PULLUP) ? GPIO_PULLUP : GPIO_OPENDRAIN;
+            stm32_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | gpio_val);
             cr |= USART_CR3_HDSEL;
           }
         else
           {
-            stm32_configgpio(priv->tx_gpio | GPIO_PUSHPULL);
+            stm32_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | GPIO_PUSHPULL);
             cr &= ~USART_CR3_HDSEL;
           }
 
diff --git a/arch/arm/src/stm32f7/stm32_serial.c b/arch/arm/src/stm32f7/stm32_serial.c
index baa1e4c877c..dbf2dac63b6 100644
--- a/arch/arm/src/stm32f7/stm32_serial.c
+++ b/arch/arm/src/stm32f7/stm32_serial.c
@@ -2125,14 +2125,15 @@ static int up_ioctl(struct file *filep, int cmd, unsigned long arg)
 
         uint32_t cr = up_serialin(priv, STM32_USART_CR3_OFFSET);
 
-        if (arg == SER_SINGLEWIRE_ENABLED)
+        if ((arg & SER_SINGLEWIRE_ENABLED) != 0)
           {
-            stm32_configgpio(priv->tx_gpio | GPIO_OPENDRAIN);
+            uint32_t gpio_val = (arg & SER_SINGLEWIRE_PULLUP) ? GPIO_PULLUP : GPIO_OPENDRAIN;
+            stm32_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | gpio_val);
             cr |= USART_CR3_HDSEL;
           }
         else
           {
-            stm32_configgpio(priv->tx_gpio | GPIO_PUSHPULL);
+            stm32_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | GPIO_PUSHPULL);
             cr &= ~USART_CR3_HDSEL;
           }
 
diff --git a/arch/arm/src/stm32h7/stm32_serial.c b/arch/arm/src/stm32h7/stm32_serial.c
index fe59bdd2266..942bc15028d 100644
--- a/arch/arm/src/stm32h7/stm32_serial.c
+++ b/arch/arm/src/stm32h7/stm32_serial.c
@@ -1529,14 +1529,15 @@ static int up_ioctl(struct file *filep, int cmd, unsigned long arg)
 
         uint32_t cr = up_serialin(priv, STM32_USART_CR3_OFFSET);
 
-        if (arg == SER_SINGLEWIRE_ENABLED)
+        if ((arg & SER_SINGLEWIRE_ENABLED) != 0)
           {
-            stm32_configgpio(priv->tx_gpio | GPIO_OPENDRAIN);
+            uint32_t gpio_val = (arg & SER_SINGLEWIRE_PULLUP) ? GPIO_PULLUP : GPIO_OPENDRAIN;
+            stm32_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | gpio_val);
             cr |= USART_CR3_HDSEL;
           }
         else
           {
-            stm32_configgpio(priv->tx_gpio | GPIO_PUSHPULL);
+            stm32_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | GPIO_PUSHPULL);
             cr &= ~USART_CR3_HDSEL;
           }
 
diff --git a/arch/arm/src/stm32l4/stm32l4_serial.c b/arch/arm/src/stm32l4/stm32l4_serial.c
index f7e2558559a..45dc13ae0e1 100644
--- a/arch/arm/src/stm32l4/stm32l4_serial.c
+++ b/arch/arm/src/stm32l4/stm32l4_serial.c
@@ -1805,14 +1805,15 @@ static int stm32l4serial_ioctl(FAR struct file *filep, int cmd,
 
         uint32_t cr = stm32l4serial_getreg(priv, STM32L4_USART_CR3_OFFSET);
 
-        if (arg == SER_SINGLEWIRE_ENABLED)
+        if ((arg & SER_SINGLEWIRE_ENABLED) != 0)
           {
-            stm32l4_configgpio(priv->tx_gpio | GPIO_OPENDRAIN);
+            uint32_t gpio_val = (arg & SER_SINGLEWIRE_PULLUP) ? GPIO_PULLUP : GPIO_OPENDRAIN;
+            stm32l4_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | gpio_val);
             cr |= USART_CR3_HDSEL;
           }
         else
           {
-            stm32l4_configgpio(priv->tx_gpio | GPIO_PUSHPULL);
+            stm32l4_configgpio((priv->tx_gpio & ~(GPIO_PUPD_MASK | GPIO_OPENDRAIN)) | GPIO_PUSHPULL);
             cr &= ~USART_CR3_HDSEL;
           }
 
diff --git a/include/nuttx/serial/tioctl.h b/include/nuttx/serial/tioctl.h
index 614b126984b..e8dd02d3072 100644
--- a/include/nuttx/serial/tioctl.h
+++ b/include/nuttx/serial/tioctl.h
@@ -185,6 +185,7 @@
 #define TIOCGSINGLEWIRE _TIOC(0x0031)  /* Get single-wire mode */
 
 #  define SER_SINGLEWIRE_ENABLED   (1 << 0) /* Enable/disable single-wire support */
+#  define SER_SINGLEWIRE_PULLUP    (1 << 1) /* Enable Pull-up on TX (Open-Drain otherwise) */
 
 /* Debugging */