diff --git a/arch/arm/src/efm32/efm32_usbdev.c b/arch/arm/src/efm32/efm32_usbdev.c
index 958d3973720..b083080be87 100644
--- a/arch/arm/src/efm32/efm32_usbdev.c
+++ b/arch/arm/src/efm32/efm32_usbdev.c
@@ -1336,6 +1336,19 @@ static void efm32_epin_request(struct efm32_usbdev_s *priv,
           empmsk |= USB_DIEPEMPMSK(privep->epphy);
           efm32_putreg(empmsk, EFM32_USB_DIEPEMPMSK);
 
+#ifdef CONFIG_DEBUG_FEATURES
+          /* Check if the configured TXFIFO size is sufficient for a given
+           * request. If not, raise an assertion here.
+           */
+
+          regval = emf32_putreg(regval, EMF32_USB_DIEPTXF(privep->epphy));
+          regval &= _USB_DIEPTXF1_INEPNTXFDEP_MASK;
+          regval >>= _USB_DIEPTXF1_INEPNTXFDEP_SHIFT;
+          uerr("EP%" PRId8 " TXLEN=%" PRId32 " nwords=%d\n",
+               privep->epphy, regval, nwords);
+          DEBUGASSERT(regval >= nwords);
+#endif
+
           /* Terminate the transfer.  We will try again when the TxFIFO empty
            * interrupt is received.
            */
diff --git a/arch/arm/src/stm32/stm32_otgfsdev.c b/arch/arm/src/stm32/stm32_otgfsdev.c
index a0a9ecb70e2..229d743e943 100644
--- a/arch/arm/src/stm32/stm32_otgfsdev.c
+++ b/arch/arm/src/stm32/stm32_otgfsdev.c
@@ -1378,6 +1378,19 @@ static void stm32_epin_request(struct stm32_usbdev_s *priv,
           empmsk |= OTGFS_DIEPEMPMSK(privep->epphy);
           stm32_putreg(empmsk, STM32_OTGFS_DIEPEMPMSK);
 
+#ifdef CONFIG_DEBUG_FEATURES
+          /* Check if the configured TXFIFO size is sufficient for a given
+           * request. If not, raise an assertion here.
+           */
+
+          regval = stm32_putreg(regval, STM32_OTGFS_DIEPTXF(privep->epphy));
+          regval &= OTGFS_DIEPTXF_INEPTXFD_MASK;
+          regval >>= OTGFS_DIEPTXF_INEPTXFD_SHIFT;
+          uerr("EP%" PRId8 " TXLEN=%" PRId32 " nwords=%d\n",
+               privep->epphy, regval, nwords);
+          DEBUGASSERT(regval >= nwords);
+#endif
+
           /* Terminate the transfer.  We will try again when the TxFIFO empty
            * interrupt is received.
            */
diff --git a/arch/arm/src/stm32/stm32_otghsdev.c b/arch/arm/src/stm32/stm32_otghsdev.c
index f16622ae537..045d2722db4 100644
--- a/arch/arm/src/stm32/stm32_otghsdev.c
+++ b/arch/arm/src/stm32/stm32_otghsdev.c
@@ -1328,6 +1328,19 @@ static void stm32_epin_request(struct stm32_usbdev_s *priv,
           empmsk |= OTGHS_DIEPEMPMSK(privep->epphy);
           stm32_putreg(empmsk, STM32_OTGHS_DIEPEMPMSK);
 
+#ifdef CONFIG_DEBUG_FEATURES
+          /* Check if the configured TXFIFO size is sufficient for a given
+           * request. If not, raise an assertion here.
+           */
+
+          regval = stm32_putreg(regval, STM32_OTGHS_DIEPTXF(privep->epphy));
+          regval &= OTGHS_DIEPTXF_INEPTXFD_MASK;
+          regval >>= OTGHS_DIEPTXF_INEPTXFD_SHIFT;
+          uerr("EP%" PRId8 " TXLEN=%" PRId32 " nwords=%d\n",
+               privep->epphy, regval, nwords);
+          DEBUGASSERT(regval >= nwords);
+#endif
+
           /* Terminate the transfer.  We will try again when the TxFIFO empty
            * interrupt is received.
            */
diff --git a/arch/arm/src/stm32f7/stm32_otgdev.c b/arch/arm/src/stm32f7/stm32_otgdev.c
index 93ed4b5ff6e..cee9ad13fbf 100644
--- a/arch/arm/src/stm32f7/stm32_otgdev.c
+++ b/arch/arm/src/stm32f7/stm32_otgdev.c
@@ -1438,6 +1438,19 @@ static void stm32_epin_request(struct stm32_usbdev_s *priv,
           empmsk |= OTG_DIEPEMPMSK(privep->epphy);
           stm32_putreg(empmsk, STM32_OTG_DIEPEMPMSK);
 
+#ifdef CONFIG_DEBUG_FEATURES
+          /* Check if the configured TXFIFO size is sufficient for a given
+           * request. If not, raise an assertion here.
+           */
+
+          regval = stm32_putreg(regval, STM32_OTG_DIEPTXF(privep->epphy));
+          regval &= OTG_DIEPTXF_INEPTXFD_MASK;
+          regval >>= OTG_DIEPTXF_INEPTXFD_SHIFT;
+          uerr("EP%" PRId8 " TXLEN=%" PRId32 " nwords=%d\n",
+               privep->epphy, regval, nwords);
+          DEBUGASSERT(regval >= nwords);
+#endif
+
           /* Terminate the transfer.  We will try again when the TxFIFO empty
            * interrupt is received.
            */
diff --git a/arch/arm/src/stm32h7/stm32_otgdev.c b/arch/arm/src/stm32h7/stm32_otgdev.c
index 7a72b5ec2b6..e685f6501d8 100644
--- a/arch/arm/src/stm32h7/stm32_otgdev.c
+++ b/arch/arm/src/stm32h7/stm32_otgdev.c
@@ -1401,6 +1401,19 @@ static void stm32_epin_request(struct stm32_usbdev_s *priv,
           empmsk |= OTG_DIEPEMPMSK(privep->epphy);
           stm32_putreg(empmsk, STM32_OTG_DIEPEMPMSK);
 
+#ifdef CONFIG_DEBUG_FEATURES
+          /* Check if the configured TXFIFO size is sufficient for a given
+           * request. If not, raise an assertion here.
+           */
+
+          regval = stm32_putreg(regval, STM32_OTG_DIEPTXF(privep->epphy));
+          regval &= OTG_DIEPTXF_INEPTXFD_MASK;
+          regval >>= OTG_DIEPTXF_INEPTXFD_SHIFT;
+          uerr("EP%" PRId8 " TXLEN=%" PRId32 " nwords=%d\n",
+               privep->epphy, regval, nwords);
+          DEBUGASSERT(regval >= nwords);
+#endif
+
           /* Terminate the transfer.  We will try again when the TxFIFO empty
            * interrupt is received.
            */
diff --git a/arch/arm/src/stm32l4/stm32l4_otgfsdev.c b/arch/arm/src/stm32l4/stm32l4_otgfsdev.c
index b5f5f5314cc..44d3fec6808 100644
--- a/arch/arm/src/stm32l4/stm32l4_otgfsdev.c
+++ b/arch/arm/src/stm32l4/stm32l4_otgfsdev.c
@@ -1438,6 +1438,20 @@ static void stm32l4_epin_request(struct stm32l4_usbdev_s *priv,
           empmsk |= OTGFS_DIEPEMPMSK(privep->epphy);
           stm32l4_putreg(empmsk, STM32L4_OTGFS_DIEPEMPMSK);
 
+#ifdef CONFIG_DEBUG_FEATURES
+          /* Check if the configured TXFIFO size is sufficient for a given
+           * request. If not, raise an assertion here.
+           */
+
+          regval = stm32l4_putreg(regval,
+                                  STM32L4_OTG_DIEPTXF(privep->epphy));
+          regval &= OTGFS_DIEPTXF_INEPTXFD_MASK;
+          regval >>= OTGFS_DIEPTXF_INEPTXFD_SHIFT;
+          uerr("EP%" PRId8 " TXLEN=%" PRId32 " nwords=%d\n",
+               privep->epphy, regval, nwords);
+          DEBUGASSERT(regval >= nwords);
+#endif
+
           /* Terminate the transfer.  We will try again when the TxFIFO empty
            * interrupt is received.
            */