diff --git a/esphome/components/mcp23016/__init__.py b/esphome/components/mcp23016/__init__.py
index 5a1f011617..b71d57498a 100644
--- a/esphome/components/mcp23016/__init__.py
+++ b/esphome/components/mcp23016/__init__.py
@@ -5,6 +5,7 @@ import esphome.config_validation as cv
 from esphome.const import (
     CONF_ID,
     CONF_INPUT,
+    CONF_INTERRUPT_PIN,
     CONF_INVERTED,
     CONF_MODE,
     CONF_NUMBER,
@@ -24,6 +25,7 @@ CONFIG_SCHEMA = (
     cv.Schema(
         {
             cv.Required(CONF_ID): cv.declare_id(MCP23016),
+            cv.Optional(CONF_INTERRUPT_PIN): pins.internal_gpio_input_pin_schema,
         }
     )
     .extend(cv.COMPONENT_SCHEMA)
@@ -35,6 +37,8 @@ async def to_code(config):
     var = cg.new_Pvariable(config[CONF_ID])
     await cg.register_component(var, config)
     await i2c.register_i2c_device(var, config)
+    if interrupt_pin := config.get(CONF_INTERRUPT_PIN):
+        cg.add(var.set_interrupt_pin(await cg.gpio_pin_expression(interrupt_pin)))
 
 
 def validate_mode(value):
diff --git a/esphome/components/mcp23016/mcp23016.cpp b/esphome/components/mcp23016/mcp23016.cpp
index fbdb6903b8..118a77ce37 100644
--- a/esphome/components/mcp23016/mcp23016.cpp
+++ b/esphome/components/mcp23016/mcp23016.cpp
@@ -24,11 +24,22 @@ void MCP23016::setup() {
 
   // all pins input
   this->write_reg_(MCP23016_IODIR1, 0xFFFF);
+
+  if (this->interrupt_pin_ != nullptr) {
+    this->interrupt_pin_->setup();
+    this->interrupt_pin_->attach_interrupt(&MCP23016::gpio_intr, this, gpio::INTERRUPT_FALLING_EDGE);
+    this->set_invalidate_on_read_(false);
+  }
+  this->disable_loop();
 }
 
+void IRAM_ATTR MCP23016::gpio_intr(MCP23016 *arg) { arg->enable_loop_soon_any_context(); }
 void MCP23016::loop() {
   // Invalidate cache at the start of each loop
   this->reset_pin_cache_();
+  if (this->interrupt_pin_ != nullptr) {
+    this->disable_loop();
+  }
 }
 bool MCP23016::digital_read_hw(uint8_t pin) { return this->read_reg_(MCP23016_GP1, &this->input_mask_); }
 
@@ -37,6 +48,9 @@ void MCP23016::digital_write_hw(uint8_t pin, bool value) { this->update_reg_(pin
 void MCP23016::pin_mode(uint8_t pin, gpio::Flags flags) {
   if (flags == gpio::FLAG_INPUT) {
     this->update_reg_(pin, true, MCP23016_IODIR1);
+    if (this->interrupt_pin_ == nullptr) {
+      this->enable_loop();
+    }
   } else if (flags == gpio::FLAG_OUTPUT) {
     this->update_reg_(pin, false, MCP23016_IODIR1);
   }
diff --git a/esphome/components/mcp23016/mcp23016.h b/esphome/components/mcp23016/mcp23016.h
index 494bc9c197..32149ba3e2 100644
--- a/esphome/components/mcp23016/mcp23016.h
+++ b/esphome/components/mcp23016/mcp23016.h
@@ -35,7 +35,10 @@ class MCP23016 : public Component, public i2c::I2CDevice, public gpio_expander::
 
   float get_setup_priority() const override;
 
+  void set_interrupt_pin(InternalGPIOPin *pin) { this->interrupt_pin_ = pin; }
+
  protected:
+  static void IRAM_ATTR gpio_intr(MCP23016 *arg);
   // Virtual methods from CachedGpioExpander
   bool digital_read_hw(uint8_t pin) override;
   bool digital_read_cache(uint8_t pin) override;
@@ -51,6 +54,7 @@ class MCP23016 : public Component, public i2c::I2CDevice, public gpio_expander::
   uint16_t olat_{0x0000};
   // Cache for input values (16-bit combined for both banks)
   uint16_t input_mask_{0x0000};
+  InternalGPIOPin *interrupt_pin_{nullptr};
 };
 
 class MCP23016GPIOPin : public GPIOPin {
diff --git a/tests/components/mcp23016/common.yaml b/tests/components/mcp23016/common.yaml
index e8e3ad9d08..81f38b3f52 100644
--- a/tests/components/mcp23016/common.yaml
+++ b/tests/components/mcp23016/common.yaml
@@ -1,6 +1,10 @@
 mcp23016:
-  i2c_id: i2c_bus
-  id: mcp23016_hub
+  - i2c_id: i2c_bus
+    id: mcp23016_hub
+  - i2c_id: i2c_bus
+    id: mcp23016_hub_int
+    address: 0x21
+    interrupt_pin: ${interrupt_pin}
 
 binary_sensor:
   - platform: gpio
diff --git a/tests/components/mcp23016/test.esp32-idf.yaml b/tests/components/mcp23016/test.esp32-idf.yaml
index b47e39c389..8c3b341dce 100644
--- a/tests/components/mcp23016/test.esp32-idf.yaml
+++ b/tests/components/mcp23016/test.esp32-idf.yaml
@@ -1,3 +1,6 @@
+substitutions:
+  interrupt_pin: GPIO15
+
 packages:
   i2c: !include ../../test_build_components/common/i2c/esp32-idf.yaml
 
diff --git a/tests/components/mcp23016/test.esp8266-ard.yaml b/tests/components/mcp23016/test.esp8266-ard.yaml
index 4a98b9388a..69b243bfd8 100644
--- a/tests/components/mcp23016/test.esp8266-ard.yaml
+++ b/tests/components/mcp23016/test.esp8266-ard.yaml
@@ -1,3 +1,6 @@
+substitutions:
+  interrupt_pin: GPIO15
+
 packages:
   i2c: !include ../../test_build_components/common/i2c/esp8266-ard.yaml
 
diff --git a/tests/components/mcp23016/test.rp2040-ard.yaml b/tests/components/mcp23016/test.rp2040-ard.yaml
index 319a7c71a6..b8ad1e4792 100644
--- a/tests/components/mcp23016/test.rp2040-ard.yaml
+++ b/tests/components/mcp23016/test.rp2040-ard.yaml
@@ -1,3 +1,6 @@
+substitutions:
+  interrupt_pin: GPIO2
+
 packages:
   i2c: !include ../../test_build_components/common/i2c/rp2040-ard.yaml