diff --git a/esphome/components/esp32/helpers.cpp b/esphome/components/esp32/helpers.cpp
index 051b7ce162..76f1c59c73 100644
--- a/esphome/components/esp32/helpers.cpp
+++ b/esphome/components/esp32/helpers.cpp
@@ -20,12 +20,6 @@ bool random_bytes(uint8_t *data, size_t len) {
   return true;
 }
 
-Mutex::Mutex() { handle_ = xSemaphoreCreateMutex(); }
-Mutex::~Mutex() {}
-void Mutex::lock() { xSemaphoreTake(this->handle_, portMAX_DELAY); }
-bool Mutex::try_lock() { return xSemaphoreTake(this->handle_, 0) == pdTRUE; }
-void Mutex::unlock() { xSemaphoreGive(this->handle_); }
-
 // only affects the executing core
 // so should not be used as a mutex lock, only to get accurate timing
 IRAM_ATTR InterruptLock::InterruptLock() { portDISABLE_INTERRUPTS(); }
diff --git a/esphome/components/esp8266/helpers.cpp b/esphome/components/esp8266/helpers.cpp
index 4a64ae181e..aadfc31197 100644
--- a/esphome/components/esp8266/helpers.cpp
+++ b/esphome/components/esp8266/helpers.cpp
@@ -12,12 +12,8 @@ namespace esphome {
 uint32_t random_uint32() { return os_random(); }
 bool random_bytes(uint8_t *data, size_t len) { return os_get_random(data, len) == 0; }
 
-// ESP8266 doesn't have mutexes, but that shouldn't be an issue as it's single-core and non-preemptive OS.
-Mutex::Mutex() {}
-Mutex::~Mutex() {}
-void Mutex::lock() {}
-bool Mutex::try_lock() { return true; }
-void Mutex::unlock() {}
+// ESP8266 Mutex is defined inline as a no-op in helpers.h when USE_ESP8266 (or USE_RP2040) is set,
+// independent of the ESPHOME_THREAD_SINGLE thread model define.
 
 IRAM_ATTR InterruptLock::InterruptLock() { state_ = xt_rsil(15); }
 IRAM_ATTR InterruptLock::~InterruptLock() { xt_wsr_ps(state_); }
diff --git a/esphome/components/libretiny/helpers.cpp b/esphome/components/libretiny/helpers.cpp
index 21913e4a16..ffbd181c54 100644
--- a/esphome/components/libretiny/helpers.cpp
+++ b/esphome/components/libretiny/helpers.cpp
@@ -15,12 +15,6 @@ bool random_bytes(uint8_t *data, size_t len) {
   return true;
 }
 
-Mutex::Mutex() { handle_ = xSemaphoreCreateMutex(); }
-Mutex::~Mutex() {}
-void Mutex::lock() { xSemaphoreTake(this->handle_, portMAX_DELAY); }
-bool Mutex::try_lock() { return xSemaphoreTake(this->handle_, 0) == pdTRUE; }
-void Mutex::unlock() { xSemaphoreGive(this->handle_); }
-
 // only affects the executing core
 // so should not be used as a mutex lock, only to get accurate timing
 IRAM_ATTR InterruptLock::InterruptLock() { portDISABLE_INTERRUPTS(); }
diff --git a/esphome/components/rp2040/helpers.cpp b/esphome/components/rp2040/helpers.cpp
index 4191c2164a..a69b8da480 100644
--- a/esphome/components/rp2040/helpers.cpp
+++ b/esphome/components/rp2040/helpers.cpp
@@ -35,12 +35,7 @@ bool random_bytes(uint8_t *data, size_t len) {
   return true;
 }
 
-// RP2040 doesn't have mutexes, but that shouldn't be an issue as it's single-core and non-preemptive OS.
-Mutex::Mutex() {}
-Mutex::~Mutex() {}
-void Mutex::lock() {}
-bool Mutex::try_lock() { return true; }
-void Mutex::unlock() {}
+// RP2040 Mutex is defined inline in helpers.h for RP2040/ESP8266 builds.
 
 IRAM_ATTR InterruptLock::InterruptLock() { state_ = save_and_disable_interrupts(); }
 IRAM_ATTR InterruptLock::~InterruptLock() { restore_interrupts(state_); }
diff --git a/esphome/core/helpers.h b/esphome/core/helpers.h
index c2f4cace9a..d220626bcf 100644
--- a/esphome/core/helpers.h
+++ b/esphome/core/helpers.h
@@ -1866,19 +1866,34 @@ template<typename T> class Parented {
  */
 class Mutex {
  public:
-  Mutex();
   Mutex(const Mutex &) = delete;
+  Mutex &operator=(const Mutex &) = delete;
+
+#if defined(USE_ESP8266) || defined(USE_RP2040)
+  // Single-threaded platforms: inline no-ops so the compiler eliminates all call overhead.
+  Mutex() = default;
+  ~Mutex() = default;
+  void lock() {}
+  bool try_lock() { return true; }
+  void unlock() {}
+#elif defined(USE_ESP32) || defined(USE_LIBRETINY)
+  // FreeRTOS platforms: inline to avoid out-of-line call overhead.
+  Mutex() { handle_ = xSemaphoreCreateMutex(); }
+  ~Mutex() = default;
+  void lock() { xSemaphoreTake(this->handle_, portMAX_DELAY); }
+  bool try_lock() { return xSemaphoreTake(this->handle_, 0) == pdTRUE; }
+  void unlock() { xSemaphoreGive(this->handle_); }
+
+ private:
+  SemaphoreHandle_t handle_;
+#else
+  Mutex();
   ~Mutex();
   void lock();
   bool try_lock();
   void unlock();
 
-  Mutex &operator=(const Mutex &) = delete;
-
  private:
-#if defined(USE_ESP32) || defined(USE_LIBRETINY)
-  SemaphoreHandle_t handle_;
-#else
   // d-pointer to store private data on new platforms
   void *handle_;  // NOLINT(clang-diagnostic-unused-private-field)
 #endif